Studiy for inhibition of Tomato spotted wilt virus by ecologically pure product extract of Leuzea carthamoides

Bistra Dikova, Milena Nikolova, Anatoli Djourmanski

Abstract: The virus diseases cause yearly losses in the quantity and quality of the production of pepper. Ecologically clean means safe for human health that can inhibit plant viruses are necessary for keeping of clean environments and healthy people. Such means are extracts from medicinal plants, essential oils and others. The object of present study is leuzea - maral root [Leuzea carthamoides (Willd) DC]. Dried powdered root material from Leuzea was extracted with 80% methanol by maceration. After evaporation of the solvent the crude extract was subjected to subsequent analysis. The inhibiting effect of the extract of L. carthamoides was studied on pepper against economically important virus for vegetables, as Тomato spotted wilt virus (TSWV). The tests were carried out 2018 and 2019 in different concentrations of the diluted with distilled water extract – 1000 ppm, 5000 рpm, 10000 ppm, 15000 ppm и 20000 ppm by using immune enzyme method ELISA, variant DAS ELISA. The results showed, that the dilution of the L. carthamoides extract in concentration 20000 ppm in the trials „in vitro“ is suitable for use, because the extinction values for TSWV were negative i.e. inhibiting effect against TSWV was established. The tests „in vivo” in 2019 showed also 20000 ppm was a concentration with a good inhibiting effect against TSWV without one phytotoxic effect on the pepper plants. Metabolic profile of studied extract was analyzed by GC-MS. A variety of fatty, organic and phenolic acids, alkanes, fatty alcohols, mono- and disaccharides were identified. Among fatty acids palmitic acid (C16:0) and linoleic acid (C18:2) were the most abundant. Chlorgenic, syringic, quinic and caffeic acids were found as free phenolic acids. Additionally after alkaline hydrolysis of methanolic extract vanilic, protocatechuic and (p) hydroxycinnamic acids were established.

Keywords: antiphytoviral effect; GC-MS, phenolic acids; Leuzea carthamoides; pepper; TSWV

Date published: 2020-03-10