Changes in the androgenic response of triticale (xTriticosecale Wittm.) as a result of the inclusion of sweet potato extract in the induction media

Hristo Stoyanov, Ivan Belchev, Valentin Baytchev

Abstract: The anther culture is a fast method of stabilizing the genetic diversity in triticale breeding. At the same time, the large number of recovered albino regenerants creates practical difficulties. The type and composition of the induction medium influences the yield of green and albino regenerants. In order to study the effect of an induction medium with sweet potato extract included, 7500 anthers of six hybrid Triticale combinations of Akord cultivar were set. 5183 calluses and embryoids were formed with a mean callus induction of 68.5%. 467 (6.2%) green and 864 (11.5%) albino plants were regenerated. The induction medium with sweet potato extract significantly reduces callus induction as well as the subsequent regeneration of green and albino plants. Combining the two extracts (potato and sweet potato) does not have significant effect on the reaction in anther culture. The use of sweet potatoextract is relatively suitable for practical purposes, mainly because of the significantly reduced production of albino regenerants.

Keywords: albino regenerants; anther culture; induction media; sweet potato extract; triticale

Date published: 2019-10-31