Investigation on biological properties of Prunus Necrotic Ringspot Virus isolates from different stone fruit species

Anelia Borisova

Abstract: The biological properties of five Bulgarian isolates of Prunus necrotic ringspot virus (PNRSV) originated from sweet cherry, sour cherry, peach and plum, identified by ELISA, were studied during the period 2013 – 2014. Тhe virus isolates were transmitted from naturally infected fruit trees to two woody species Prunus mahaleb and Prunus аvium by chip budding. Six herbaceous plant species Cucumis sativum cv. Gergana, Levina, Cornichon de Paris, Cucurbita pepo var. giromontia cv. Izobilna, Chenopodium quinoa, Helianthus annuus, Lupinus polyphyllus and Zinnia elegans were mechanically inoculated with sap from
infected young leaves of the investigated isolates. From the studied isolates the most virulent, causing cleanly severe symptoms on indicator plants was
peach isolate PhM. The sour cherry isolate SChS was caused moderate symptoms. The plum isolate PlM and sweet cherry isolates ChS and ChМ developed mild reaction. P. avium was the best host plant to detect Bulgarian PNRSV isolates. Herbaceous species Ch. quinoa, H. annuus, L. polyphyllus and Z. elegans were not susceptible to the investigated PNRSV isolates.

Keywords: ELISA; host range; PNRSV isolates; Prunus species; virus indexing

Date published: 2017-08-09