Factors affecting Camelia japonica L. clonal micropropagation

Valentina Malyarovskaya

Abstract: These studies aimed at studying the factors that influence the processes of organogenesis in tissue culture and organs of Camellia japonica in vitro. It is shown that high concentrations of 6-BAP – 2.5 and 3 mg/l cause rapid growth of callus tissues at the base of microcuttings. However, decrease at the concentration of phytohor¬mones: 6-BAP from 2.0 mg/l to 0.5 mg/l in combination with indole acetic acid from 0.5 mg/l to 0.1 mg/l induced the growth of microshoots in WPM medium. It is noted that, variant from the culture medium with phytohormones of cytokinins-and-adenine type (6 BAP and zeatin) at a concentration of 2.0 and 1.0 mg/l, respectively, and 0.1 mg/l of naphthyl acetic acid was the most appropriate for accelerating the growth of microshoots obtained from explants of mixed varieties while the variant of the culture medium containing 6 BAP 2, 0 mg/l, naphthyl acetic acid – 0.5 mg/l and gibberellic acid – 1.0 mg/l was the best for the cultivar Reine des Beautes.

Keywords: Camellia japonica; in vitro; micropropagation; microshoots; phytohormones

Date published: 2017-07-26