Investigating the possibilities for callus induction from walnut (Juglans regia) in vitro

Viktoria Nikolova, Dimitar Dimanov, Stefan Gandev

Abstract: The possibilities for callus induction from different plant explants of walnut (Juglans regia) in conditions in vitro have been examined. Segments of leaves and stems of young annual plants have been used as a source of plant explants. Three nutrient media have been tested, on which was inducted a callus tissue. The study was carried out in the period May – December 2016, in the laboratory for in vitro propagation at the Fruit Growing Institute – Plovdiv. The results show that a callus tissue has been produced in leaf parts on DKW nutrient medium
(Driver and Kuniyuki, 1984) with 1 mg/l BAP and 3 mg/l NAA, and K1 nutrient medium (Dimanov, 1987) with 2 mg/l BAP and 6 mg/l NAA. In both media the callus induction in leaf parts started after the 15th day. The callus induction in stem cells started after the 17th day on DKW nutrient medium.

Keywords: callus induction; in vitro; leaves; steams; walnut

Date published: 2017-02-28