Mycelial compatibility of Phomopsis helianthi (Munt.-Cvet. еt al.) isolates
Maria Petrova, Ivan Kiryakov
Abstract: In the present study, the genetic diversity of 30 isolates of the pathogen Phomopsis helianthi was studied using the mycelial compatibility groups (MCGs) method. Mycelial compatibility tests were carried out on two media - PDA + 80 μl/L and PSA +80 μl/L, with added colorant (Christmas red - Sly Commerce OOD) and without added colorant. The use of PDA medium was found to be inadvisable due to the indistinct dividing line (groove) in isolates having an incompatible phenotypic response. Based on the tests done, 416 combinations were carried out and the four types of reaction were observed- NC-N – incompatible with necrosis, NC-Gincompatible, mycelium do not approach with a pronounced distance between them, NC-DL- both lines (black) are spaced, C-compatible. Their percentage ratio is presented. Isolates are grouped into 20 MCGs, with two of the groups containing only one isolate due to their incompatibility. The remaining isolates were found to occur in more than one MCG. The isolates originating from the region of Ruse were marked with the greatest genetic diversity, and the isolates from the area of Karnobat with the least.
Keywords: genetic diversity; mycelial compatibility groups; Phomopsis helianthi
Citation: Petrova, M., & Kiryakov, I. (2024). Mycelial compatibility of Phomopsis helianthi (Munt.-Cvet. еt al.) isolates. Bulgarian Journal of Crop Science, 61(4) 16-24 (Bg).
| References: (click to open/close) | Acimovic, M., & Straser, N. (1982). Phomopsis sp. - a new parasite of sunflower. Zastita Bilja. 33(2), 117-158.
Antonova, T. S. (1999). Biological features of the development of Phomopsis / T. S. Antonova // Protection and quarantine of plants. 1., P. 25-26.
Didenko, A. O. (2015). Basic biological developments and control of the pathogen of the sunflower phomopsis in the conditions of the Krasnodar region. Ph.D. thesis. Krasnodar.
Entcheva, V. (2002). Variation in the aggressiveness of isolates from Phomopsis/Diaporthe helianthi Munt.-Cvet. et al. on sunflower. Bulgarian Journal of Agricultural Science. 8(4), 349-352.
Gulya, T., Rashid, K. Y., & Masirevic, S. M. (1997). Sunflower diseases. Sunflower technology and production. 35, 263-379.
Kamvar, Z. N., & Everhart, S. E. (2019). Something in the agar does not compute: on the discriminatory power of mycelial compatibility in Sclerotinia sclerotiorum. Tropical Plant Pathology, 44, 32-40.
Kiryakov, I., & Zhecheva, K. (2019). Mycelial compatibility and aggressiveness of Bulgarian Sclerotinia sclerotiorum isolates. Field Crops Studies, XII (3), 9-22.
Liua, J., Meng, Q., Zhang, Y., Xianga, H., Li, Y., Shi, F., Ma, L., Liu, C., Liu, Y., Su, B., & Li, Z. (2018). Mycelial compatibility group and genetic variation of sunflower Sclerotinia sclerotiorum in Northeast China. Physiological and Molecular Plant Pathology, 102: 185-192.
Masirevic, S., & Gulya, T. J. (1992). Sclerotinia and Phomopsis—two devastating sunflower pathogens. Field Crops Research, 30(3-4), 271-300.
Mathew, F. M., Rashid, K. Y., Gulya, T. J., & Markell, S. G. (2015A). First report of Phomopsis stem canker of sunflower (Helianthus annuus) caused by Diaporthe gulyae in Canada. Plant Disease, 99(1), 160-160.
Mathew, F. M., Alananbeh, K. M., Jordahl, J. G., Meyer, S. M., Castlebury, L. A., Gulya, T. J., & Markell, S. G. (2015B). Phomopsis stem canker: A reemerging threat to sunflower (Helianthus annuus) in the United States. Phytopathology, 105(7), 990-997.
Mathew, F., Olson, T., Marek, L., Gulya, T., & Markell, S. (2018). Identification of sunflower (Helianthus annuus) accessions resistant to Diaporthe helianthi and Diaporthe gulyae. Plant Health Prog. 19: 97–102.
Muntañola-Cvetković, M., Mihaljčević, M., Vukojević, J., & Petrov, M. (1985). Comparisons of Phomopsis isolates obtained from sunflower plants and debris in Yugoslavia. Transactions of the British Mycological Society, 85(3), 477-483.
Sackston, W. E. (1981). The Sunflower Crop and Disease: Progress, Problems, and Prospects. Plant disease, 65(8), 643-648.
Says-Lesage, V., Roeckel-Drevet, P., Viguié, A., Tourvieille, J., Nicolas, P., & de Labrouhe, D. T. (2002). Molecular variability within Diaporthe/Phomopsis helianthi from France. Phytopathology, 92(3), 308-313.
Schafer, M. R., & Kohn, L. M. (2006). An optimized method for mycelial compatibility testing in Sclerotinia sclerotiorum. Mycologia, 98(4), 593-597.
Scheper, R. W. A. (2001). Studies on the biology and genetic variation of phomopsis on grapevine/Reiny WA Scheper (Doctoral dissertation).
Slyusar, E., & Firaz, M. (1995). Response of wild sunflower species to Phomopsis infection. Selection and seed breeding, 1995, N 3 pp. 22-23.
Smetnik, A. I., Aleksandrov, I. N., Skripka, O. V. & Izmalkova, A. G. (1998). Sunflower seeds as a way of preservation of Phomopsis infection. Zashchita i Karantin Rasteniĭ, (1), 35-36.
Strom, N. B. & Bushley, K. E. (2016). Two genomes are better than one: history, genetics, and biotechnological applications of fungal heterokaryons. Fungal biology and biotechnology, 3(1), 1-14.
Rozhkovan, V, Tereshchenko, B. & Stus, Z. (1995). Resistance of wild sunflower species to Phomopsis. Breeding and Seed Breeding, 1995, N:3, pp. 23-26
Thompson, S. M., Tan, Y. P., Young, A. J., Neate, S. M., Aitken, E. A. B. & Shivas, R. G. (2011). Stem cankers on sunflower (Helianthus annuus) in Australia reveal a complex of pathogenic Diaporthe (Phomopsis) species. Persoonia-Molecular Phylogeny and Evolution of Fungi, 27(1), 80-89.
Vukojević, J., Franić-Mihajlović, D. & Mihaljčević, M. (1995). In vitro production of perithecia of Diaporthe helianthi. Mycopathologia, 132, 21-25.
Zivkovic, S., Stojanovic, S., Gavrilovic, V. & Ivanovic, Z. (2008). Vegetativna inkompatibilnost izolata Phomopsis spp. izolovanih sa stabla sljive.
|
|
| Date published: 2024-08-29
Download full text 
