Analysis of germination of seeds of Capsicum annum, L. and Lycorersicum esculent, L. treated with growth-promoting compounds using fluorescence spectroscopy

Vanya Slavova, Veselina Masheva, Katya Uzundzhalieva, Nurettin Tahsin

Abstract: Seed germination is a complex process caused by the interaction of hormonal, metabolic, genetic, and environmental factors. The variability of this trait has a great influence on the efficient production of seedlings and the quality of the crop. The degree of seed germination can be influenced by various pretreatments and the non-destructive measurement to determine it by fluorescence spectroscopy, which will allow the application of the method as non-invasive and fast-acting. To confirm the flexibility of the method, seeds of Capsicum annum, L and Lycorersicum esculentum, L were primed with sterile distilled water (SDW), giberilic acid (GA3) and hydrogen peroxide (H2O2). A portable spectrometer, model AvaSpec-ULS2048CL-EVO, was used for the monitoring. The results are confirmed by faster logical variations of seeds, treatment with H2O2 and GA3 medium compared to seeds, and treatment with SDW for 3 consecutive days. The method can be used in various agricultural fields and shows its potential as a rigorous sensory method for rapid selection of optimal compounds that stimulate plant growth compared to standard methods.

Keywords: fluorescence spectroscopy; germination; seeds

Date published: 2022-12-16