Видов състав на причинителите на кореново и базично гниене по хидропонно отглеждани краставици в България
Иво Янашков, Георги Пренгов, Ценко Въчев
Резюме: Оранжерийните краставици, култивирани в хидропонни системи (каменна вата) са обект на нападение от гъбоподобни и гъбни причинители на кореново и базично гниене. В най-ранни фенофази болестта се развива под формата на „сечене” на разсада, а по-късно като гниене по корените и кореновата шийка, и светлокафяво оцветяване на проводящата система в основата на стъблата. Болните растения загиват най-често по време на плододаването. Резултатите от проведеното изследване показват, че сред най-широко разпространените патогени по хидропонно отглеждани краставици у нас са представители на род Pythium – 82% от фитопатогените, изолирани от симптомни краставични растения, отглеждани хидропонно върху каменна вата. Сред изолатите от този род преобладава група G на Pythium spp., съставляваща 55.0% от общия брой патогенни изолати, получени при обследване на производствени оранжерии в страната. Останалите изолати от род Pythium се отнасят към група F (5.6%), видовете P. Ultimum (13.5%) и P. Aphanidermatum (7.9%). Като причинител на гниене по корените и базите на краставичните растения е установен и видът Fusarium oxysporum f. sp. radicis-cucumerinum. Изолатите от този вид съставляват 18.0% от общия брой идентифицирани фитопатогени. Установените видове заразяват растенията самостоятелно или съвместно, като причиняват значителни икономически загуби при производството на оранжерийно отглеждани краставици.
Ключови думи: оранжерийни краставици; каменна вата; кореново и базично гниене; коренови патогени; безпочвена система
Цитиране: Yanashkov, I., Prengov, G., & Vatchev, Tz. (2024). Species composition of root and basal rot pathogens of hydroponically grown cucumbers in Bulgaria. Bulgarian Journal of Crop Science, 61(6) 73-83 (Bg).
Литература: (click to open/close) | Amaradasa, B. S., Mei, C., He, Y., Chretien, R. L., Doss, M., Durham, T., & Lowman, S. (2024). Bio¬control potential of endophytic Pseudomonas strain IALR1619 against two Pythium species in cucumber and hydroponic lettuce. Plos one, 19(2), e0298514. Asran, A. & Abd-Elsalam, K. A. (2020). Top Three Plant Pathogenic Pythium Species. In: Rai, M., Abd-Elsalam, K.A., Ingle, A.P., (eds.) Pythium: Diagnosis, Diseases and Management. CRC Press, pp. 77-91. Booth, C. (1971). The Genus Fusarium. Commonwealth Mycological Institute, Kew, Surrey, England. Booth, C. (1977). Fusarium e Laboratory Guide to the Identification of the Major Species. Commonwealth Mycological Institute, Kew, Surrey, England. Cercauskas, R. F., Brown, J., & Ferguson, G. (2001). First report of stem and root rot ofgreenhouse cucum¬ber caused by Fusarium oxysporum f. sp. radicis-cuc¬umerinum in Ontario. Plant Disease, 85(9), 1028-1028. Chatterton, S., Jayaraman, J., & Punja, Z. K. (2008). Colonization of cucumber plants by the biocontrol fungus Clonostachys rosea f. catenulate. Biological Control, 46, pp. 267-278. Cherif, M., & Belanger, R. R. (1992).Use of potasssium silicate amendments in recirculating nutrient solutions to suppress Pythium ultimum on Long English Cucum¬ber. Plant Disease, 76, pp. 1008-1011. Ellis, M. B., & Ellis, J. P. (1997). Microfungi on Land Plants: An Identification Handbook, New Enlarged Edition. The Richmond Publisher Company Ltd, 868 p. Favrin, R. J., Rahe, J. E. &Mauza, B. (1988). Pythium spp. associated with crown rot of cucumbers in Brit¬ish Columbia green houses. Plant Disease, 72, pp. 683-687. Gams, W., Aa H.A. van der, Plaats-Niternik, A. J. van der, Samson, R. A., & Salpers J. A. (1975). CBS course of Mycology. Institute of Royal Netherlands Academy of Science and Letters, 105 p. Gull, C., Labuschagne, N., & Botha, W. J. (2004). Pythium species associated with wilt and root rot of hydroponically grown crops in South Africa. African Plant Protection, 10, pp. 109–116. Herrero, M. L., Brurberg, M. B., & Hermansen, A. (2008). First report of crown and root rot caused by Phytophthora capsici o n h ydroponically g rown c u¬cumbers in Norway. Abstract, Published Online: 11 Jun 2008, https://doi.org/10.1094/PDIS-92-7-1138C. Herrero, M. L., Hermansen A., & Elen, O. N. (2003). Occurrence of Pythium spp. and Phytophthora spp. in Norwegian greenhouses and their pathogenicity on cucumber seedlings, Journal of Phytopathology, 151, pp. 36–41. doi:10.1046/j.1439-0434.2003.00676.x Ilieva, E., Arulappan, F., & Pieters, R. (1995). Phytoph¬tora root and crown of raspberry in Bulgaria. European Journal of Plant Pathology, 101, pp. 623-626. Kröber, H. (1985). Erfahrungen mit Phytophthora de Bary und Pythium Pringsheim. Mitteilungen aus der Biolo¬gischen Bundesanstalt für Land- und Forstwirtschaft, Berlin-Dahlem, Heft 225, p. 176. Leslie, J. & Summerell, B. (2006). The Fusarium Labo¬ratory Manual. Blackwell Publishing, 388 p. Marasas, W. F. O., Nelson, P. E., & Toussoun, T. A. (1984). Toxigenic Fusarium S pecies I dentity a nd Mycotoxicology. University Park. Pennsylvania State University Press., 328 p. McCullagh, M., Utkhede, R., Menzies, J. G., Punja, Z. K., & Paulitz, T. C. (1996). Evaluation of plant growth-promoting rhizobacteria for biological control of Pythium root rot of cucumbers grown in rockwool and effects on yield. European Journal of Plant Pathol¬ogy, 102, pp. 747–755. doi: 10.1007/bf01877149 Mehrotra R. S., & Aggarwal, A. (2003). Plant Pathol¬ogy. Second edition. Tata McGraw Hill Publishing Company Limited. New Delhi, 847 p. Menzies. J., Ehret. D., Barr. C., & Koch, C. (1998). Pythium spp. Isolated from healthy roots of cucumber plants growing in hydroponic culture. Canadian Jour¬nal of Plant Pathology, 20: 125 Menzies, J. G., Ehret, D. L., Koch, C., Hall, J. W., Seifert, K. A., Bissett, J., & Barr, D. J. (2005). Fungi associated with roots of cucumber grown in differ¬ent greenhouse root substrates. Canadian Journal of Botany, 83, pp. 80–92. doi: 0.1139/b04-153. Moulin, E., Lemanceau, P., & Alabouvette, C. (1994). Pathogenicity of Pythium species on cucumber in peat-sand, rockwool, and hydroponics. European Journal of Plant Pathology, 100, pp. 3-17. Nelson, P.E., Toussoun, T.A. & Marasas, W.F.O. (1983). Fusarium Species: An Illustrated Manual for Identifi-cation. Pennsylvania State University Press, University Park, 193 p. Vakalounakis, D. J., & Chalkias, J. (2004). Survival of Fusarium oxysporum f.sp. radicis-cucumerinumin soil. Crop Protection, 23(9), pp. 871-873. Paulitz, T. (1997). Biological control of root pathogens in soilless and hydroponic systems. HortScience, 32, pp. 193–96. Paulitz, T.C., Zhou, T., & Rankin, L. (1992). Selection of rhizosphere bacteria for biological control of Pythium aphanidermatum on hydroponically grown cucumber. Biological Control, 2, pp. 226–237. doi: 10.1016/1049- 9644(92)90063-j. Postma J., Bonants P. J. M., & van Os E.A. (2001). Population dynamics of Pythium aphanidermatum in cucumber grown in closed systems, Mededelin¬gen - Universiteit Gent, Faculteit Landbouwkundige en Toegepaste Biologische Wetenschappen, 66, pp. 47–59. Postma J., Geraats B. P. J., Pastoor R., & van Elsas J. D. (2005). Characterization of the microbial community involved in the suppression of Pythium aphaniderma¬tum in cucumber grown on rockwool, Phytopathology, 95, pp. 808–818. Postma J., Stevens L. H., Wiegers G. L., Davelaar E. & Nijhuis, E. H. (2009). Biological control of Pythium aphanidermatum in cucumber with a combined ap¬plication of Lysobacter enzymogenes strain 3.1T8 and chitosan, Biological Control, 48, pp. 301–309. Postma, J., Willemsen-de Klein, M. J. E. I. M., Rattink, H., & van Os, E. A. (2001). Disease suppressive soil¬less culture systems: characterization of its microflora. Acta Horticulturae, 554, pp. 323–332. doi:10.17660/ actahortic.2001.554.35 Punja, Z. K., & Parker, M. (2000). Development of Fu¬sarium root and stem rot, a new disease on greenhouse cucumber in British Columbia, caused by Fusarium oxysporum f. sp. radicis-cucumerinum. Canadian Journal of Plant Pathology, 22, pp. 349–363. Punja, Z.K., Tirajoh, A., Collyer, D., & Ni, L. (2019). Efficacy of Bacillus subtilis strain QST 713 (Rhapsody) against four major diseases of greenhouse cucum¬bers. Crop Protection, p. 124, 104845. doi:10.1016/j. cropro.2019.104845. Rafin, C., & Tirilly, Y. (1995). Characteristics and patho¬genicity of Pythium ssp. associated with root rot of tomatoes in soilless culture in Brittany, France. Plant Pathology, 44, pp. 779-785. Rivas-García, T., González-Estrada, R. R., Chiquito- Contreras, R. G., Reyes-Pérez, J. J., González-Salas, U., Hernández-Montiel, L. G., & Murillo-Amador, B. (2020). Biocontrol of Phytopathogens under Aqua¬ponics Systems. Water, 12, 2021, 15 p. doi: 10.3390/ w12072061. Rai, M., Abd-Elsalam, K.A., & Ingle, A.P. (2020). Pythium: Diagnosis, Diseases and Management. CRC Press, 392 p. Rose, S., Yip, R., & Punja, Z. K. (2004). Biological con¬trol of Fusarium and Pythium root rots on greenhouse cucumbers grown in rockwool. Acta Horticulturae, 635, pp. 73–78. doi: 10.17660/ actahortic.2004.635.9 Stanghellini, M. E., White, J. G., Tomlinson, J. A. & Clay, C. (1988). Root rot of hydroponically grown cucumbers caused by zoospore-producing isolates of Pythium intermedium. Plant Disease, 72, pp. 358-359. Sinclair, J., & Dhingra, O. (1995). Basic Plant Pathology Methods. CRC Press, 448 p. Sutton, J.C., Sopher, C.R., Owen-Going, T.N., Liu, W., Grodzinski, B., Hall, J.C., & Benchimol, R.L. (2006). Etiology and epidemiology of Pythium root rot in hydroponic crops: current knowledge and perspec¬tives. Summa Phytopathologica, 32, pp. 307–321. doi: 10.1590/s0100-54052006000400001. Thinggaard, K., & Middelboe, A. L. (1989). Phytoph¬thora and Pythium in pot plant cultures grown on ebb and flow bench with recirculating nutrient solution. Journal of Phytopathology, 125, pp. 343-352. Tran, T. T. H. (2007). I nteractions b etween s urfactant-producing Pseudomonas and Phytophthora spp. PhD Thesis, Wageningen University, The Netherlands, 136 p. Vakalounakis, D. J. 1996). Root and stem rot of cucum¬ber caused by Fusarium oxysporum f. sp. radicis-cucumerinumf. sp. nov. Plant Dis., 80, pp. 313-316. Vakalounakis, D. J., & Chalkias, J. (2004).Survival of Fusarium oxysporum f.sp.radicis-cucumerinum in soil. Crop Protection, 23, рр. 871-873. Vallance, J., Déniel, F., Floch, G., Guérin-Dubrana, L., Blancard, D., & Rey, P. (2011). Pathogenic and benefi¬cial microorganisms in soilless cultures. Agronomy for Sustainable Development, 31, рр. 191–203. doi:10.1051/ agro/2010018. van der Gaag D. J., & Wever G. (2005). Conduciveness of different soilless growing media to Pythium root and crown rot of cucumber undernear-commercial condi¬tions. Europen Journal of Plant Pathology, 112, рр. 31–41. van der Plaats-Niterink, A. J. (1981). Monograph of the genus Pythium. Studies in Mycology, 21, рр. 1-242. Vatchev, T. D. (2007). First r eport of F usarium r oot a nd stem rot of greenhouse cucumber caused by Fusarium oxysporum f. sp. radicis-cucumerinum in Bulgaria. Bul¬garian Journal of Agricultural Science, 13, рр. 151-152. Vatchev, T. D. (2015). Fusarium root and stem rot of green¬house cucumber: aerial distribution of inoculum. Bulgar-ian Journal of Agricultural Science, 21, рр. 656-660. Waterhouse, G. M. (1968) The genus Pythium Pringsheim. Diagnoses (or descriptions) and figures from the original papers. Commonwealth Mycological Institute, 100 p. Yanashkov, I. T. (2018). Soilborne fungal pathogens of small grain cereal crops in Bulgaria: species composi¬tion and distribution, methods and means of control. Disertation work, Sofia, 168 p. Zhao, Z.H., Kusakari, S.I., Okada, K., Miyazaki, A., & Osaka, T. (2000). C ontrol of P ythium r oot r ot o n hydroponically grown cucumbers with silver-coated cloth. Bioscience, Biotechnology and Biochemistry, 64, рр. 1515–1518. doi:10.1271/bbb.64.1515. Zhou, J., Wang, M., Sun, Y., Gu, Z., Wang, R., Say¬din, A., Shen, Q., & Guo, S. (2017).Nitrate increased cucumber tolerance to Fusarium wilt by regulating fungal toxin production and distribution. Toxins, 9(3), 100. Doi.org/10.3390/toxins9030100.
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| Дата на публикуване: 2024-12-19
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